cyclin d (Boster Bio)

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Boster Bio cyclin d

Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and <t>cyclin</t> <t>D</t> in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Cyclin D, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclin+d/Anti-PCNA+Antibody/pmc12819934-58-34-38
Average 95 stars, based on 292 article reviews

cyclin d - by Bioz Stars, 2026-09

95/100 stars

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1) Product Images from "Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate"

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

Journal: Cellular and Molecular Life Sciences: CMLS

doi: 10.1007/s00018-025-06071-3

Figure Legend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Techniques Used: Western Blot, Knockdown, Negative Control

Incubation:

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate
Article Snippet: .. The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents. .. The total RNA of tissue or PASMCs samples was extracted by TRIzol reagent (Thermo Fisher, MA) according to the manufacturer’s protocol.

Article Title: Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.
Article Snippet: .. These membranes were blocked at room temperature for 1 h in blocking buffer (Tris 20 mM, pH 7.6, nacl 150 mM and Tween 20 0.1%) containing 5% nonfat dry milk and incubated with Pcna (Boster Biological Technology; cat. no. BM3888; anti-Pcna antibody; 1:400), cyclin a (Boster Biological Technology; cat. no. BM4673; anti-cyclin a2 antibody; 1:200), cyclin d (Boster Biological Technology; cat. no. BM4272; anti-cyclin d1 antibody; 1:200), cyclin e (Boster Biological Technology; cat. no. BM4658; anti-cyclin e2 antibody; 1:100), β-actin (Boster Biological Technology; cat. no. BM0627; anti-β-actin antibody; 1:2,000) at 4̊C overnight. .. Secondary antibodies [Beyotime institute of Biotechnology; cat. no. a0208; horseradish peroxidase (HrP)-labeled Goat anti-rabbit igG(H+l); 1:10,000; cat. no. a0216; HrP-labeled Goat anti-Mouse igG(H+l); 1:12,000] were prepared according to the corresponding proportion and species, and incubated at room temperature for 1 h. The proteins were visualized with enhanced chemiluminescence reagents (Super Signal; Pierce; Thermo Fisher Scientific, Inc.).

Article Title: Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation.
Article Snippet: pulmonary artery smooth muscle cell proliferation Hongyue Zhang, Ying Liu, Lixin Yan, Siqi Wang, Min Zhang, Cui Ma, Xiaodong Zheng, He Chen and Daling Zhu 1, College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, China; 2, Central Laboratory of Harbin Medical University-Daqing, Daqing, Heilongjiang, China; 3, College of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, China.. 4, College of Medical Laboratory Science and Technology, Harbin Medical UniversityDaqing, Daqing, Heilongjiang, China; 5, Department of Pathophysiology, Harbin Medical University-Daqing, Daqing, Heilongjiang, China; 6, Department of Obstetrics and gynecology, The Second affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.. Correspondence and requests for reprints should be addressed to Daling Zhu Department of Biopharmaceutical Sciences, Harbin Medical University (Daqing), Xinyang Road, Daqing, Heilongjiang 163319, China.

Article Title: Fibroblast growth factor 21 (FGF21) promotes porcine granulosa cell estradiol production and proliferation via PI3K/AKT/mTOR signaling.
Article Snippet: The proliferation and steroidogenesis of mammalian ovarian granulosa cells (GCs) are related to follicular development.. Previous studies found that fibroblast growth factor 21 (FGF21) regulated female fertility through the hypothalamicepituitaryegonad axis.. However, FGF21 receptors are expressed on GCs, so we speculate that it might affect female reproduction by regulating their physiological activities.

Blocking Assay:

other:

Article Title: Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation
Article Snippet: Antibodies against Cyclin A, Cyclin D, PCNA, UB, P62, ATG5 and ATG7 (Catalog numbers BM1582, BM4272, BM0104, BM4359, BA2849, BA3525 and BA3527) were obtained from Boster (Wuhan, China).

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